Representative data for ERK/AKT/p70 S6K Activation InstantOne™ ELISA. Left: A431 cells were pre-treated with 10 µM AG1478 (an EGFR kinase inhibitor), washed and unstimulated (-) or stimulated with 10 ng/mL EGF for 10 min (+). Cells were analyzed for phosphorylated ERK 1/2/3 by InstantOne™ ELISA. Middle and Right: MCF7 cells were pre-treated with 10 µM wortmannin washed and unstimulated (-) or stimulated with 10 ng/mL insulin for 10 min (+). Cells were analyzed for phosphorylated AKT 1/2/3 (middle) and p70 S6K (right) by InstantOne™ ELISA.